Multiple displacement amplification products are compatible with recombination-based cloning

Multiple displacement amplification products are compatible with recombination-based cloning.

Genome amplification of single sperm using multiple displacement amplification

Sperm typing is an effective way to study recombination rate on a fine scale in regions of interest. There are two strategies for the amplification of single meiotic recombinants: repulsion-phase allele-specific PCR and whole genome amplification (WGA). The former can selectively amplify single recombinant molecules from a batch of sperm but is not scalable for high-throughput operation. Curren...

Enhanced sequencing coverage with digital droplet multiple displacement amplification

Sequencing small quantities of DNA is important for applications ranging from the assembly of uncultivable microbial genomes to the identification of cancer-associated mutations. To obtain sufficient quantities of DNA for sequencing, the small amount of starting material must be amplified significantly. However, existing methods often yield errors or non-uniform coverage, reducing sequencing da...

Correction: Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Single-Cell Sequencing

[This corrects the article DOI: 10.1371/journal.pone.0114520.].

Fluorogenic bidirectional displacement probe-based real-time isothermal DNA amplification and specific visual detection of products.

A unique fluorogenic nucleic acid probe has been devised, called the fluorogenic bidirectional displacement probe, which can serve as both the primer and the signal indicator of amplification products for the development of the real-time isothermal DNA amplification and its visual detection of products with high sensitivity and specificity.